First, usage a wax pencil to draw a circle on the microscope slide to separate each kind of bacteria the is walk to it is in sampled.

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If a straightforward stain of only one type of bacteria, or single mixed sample of bacteria is gift prepared, no separation is required.
Article Summary: In bespeak to watch individual bacteria v a light microscope, a bacterial smear must be attached come a slide and then stained. Right here is the procedure.
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In stimulate to warmth fix a bacter smear, it is vital to very first let the bacter sample waiting dry. Climate either place the on slide in the on slide holder that a microincinerator, or pass the dried slide with the fire of a Bunsen burner 3 or 4 times, smear side encountering up. When the on slide is heat fixed, it can then be stained.
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In stimulate to be able to clearly see individual bacteria, a sample the a bacterial colony must be mixed into water or physiological saline. This helps to evenly spread out out the bacterial sample.

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Bacterial smear after warmth fixing and also before staining. Positive bacterial control in one on the left, negative control ~ above right, and unknown bacteria in center circle. 
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